Genetic labels for cryo-Electron Microscopy
In addition to the suite of bioengineered EM labels for volume EM (EMcapsulins) optimized for heavy metal staining, we are also developing customized labels for correlative cryo-electron tomography (cryo-ET) workflows. This bioengineering work, funded by a Chan Zuckerberg Initiative (CZI) grant, will enable identification of subcellular components and labeling of subcomponents in macromolecular cellular complexes.
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Publications:
Sigmund, F., Massner, C., Erdmann, P. et al. Bacterial encapsulins as orthogonal compartments for mammalian cell engineering. Nat Commun 9, 1990 (2018). https://doi.org/10.1038/s41467-018-04227-3
Sigmund, F., Pettinger, S., Kube, M. et al. Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene Reporters ACS Nano 2019 13 (7), 8114-8123. https://doi.org/10.1021/acsnano.9b03140
Farhadi, A., Sigmund, F., Westmeyer, G.G. et al. Genetically encodable materials for non-invasive biological imaging. Nat. Mater. 20, 585–592 (2021). https://doi.org/10.1038/s41563-020-00883-3
Sigmund, F., Berezin, O., Beliakova, S. et al. Genetically encoded barcodes for correlative volume electron microscopy. Nat Biotechnol 41, 1734–1745 (2023). https://doi.org/10.1038/s41587-023-01713-yion:
Contact
Gil Westmeyer
Helmholtz Munich
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